As a dietary supplement, beta-hydroxybutyrate needs to bind to a mineral such as sodium prior to consumption. Mineral bonds improve BHB absorption and facilitate its transport across the gut-blood barrier. Once ingested, the body separates the sodium from the beta-hydroxybutyrate. While the body can produce beta-hydroxybutyrate on its own, the initial phase of ketogenic diets often creates a deficit. The body often struggles to catch up with the sudden demand for ketones. By giving the body an abundant supply of ketones while it adjusts, individuals may experience several health benefits.
This test measures the level of beta-hydroxybutyrate (BHB) in the blood. B-hydroxybutyrate is one of several chemicals known as ketones. Ketones are produced when the body is not able to utilize glucose (sugar) as energy either because of low blood sugar, insufficient insulin, or an inability to properly utilize insulin. When glucose is unavailable, the body will use fat as an alternative source of energy. Ketones are produced as fat is metabolized. The excessive buildup us ketones in the blood can cause a condition know as ketoacidosis. The body will attempt to flush the excess ketones which can cause increased urination, loss of electrolytes, and dehydration. B-hydroxybutyrate is the most prevalent ketone is cases of ketoacidosis. Diabetic Ketoacidosis is usually associated with type 1 diabetes and rarely with type 2. It can be life-threatening if not treated quickly. Ketoacidosis should not be confused with ketosis, a condition associated with low carbohydrate diets and weight loss plans.
Beta hydroxybutyrate, BHB, can help fuel the heart, muscles, and brain during periods of low carbohydrate intake. Its energy-boosting properties can aid in prolonged exercise and mental function. Sodium combined with BHB may help with muscle maintenance, nerve function, and fluid balance.
What is beta hydroxybutyrate used for in the body Some of the most noteworthy beta hydroxybutyrate benefits and uses include supporting weight loss, preventing or treating diabetes and insulin resistance, helping to treat epilepsy, fighting cancer, protecting the brain, improving cognitive function, and potentially increasing life span/longevity.
The definition of beta hydroxybutyrate is a ketone body (or simply a ketone) that is an intermediate product of the breakdown of fats in the body. Ketogenesis is considered the metabolism of fatty acids by β-oxidation. BHB is one of three main ketone body compounds that are produced when someone is in the metabolic state of ketosis (the other two are acetoacetic acid and acetone).
Other situations in which beta hydroxybutyrate is produced is when someone fasts (abstains from eating food) for around 16 hours or more. Since the body interprets fasting as a sign that energy intake is low, it increases ketone production in order to have another fuel source. The production of ketone bodies helps save any available glucose for the brain, which uses glucose quickly. When glucose is not available, fatty acids as well as ketone bodies can also be metabolized by the brain.
Exogenous ketones (ketones supplements that come from outside the body) are also used to mimic or amplify the positive effects of calorie restriction or very low carbohydrate ketogenic diets. While there are three types of ketone bodies, the ketone found in exogenous ketone supplements is usually only beta hydroxybutyrate.
The best way to naturally increase/optimize beta hydroxybutyrate levels is by eating a very-low carb, very high-fat diet (the ketogenic diet). This can also be combined with intermittent fasting or other types of fasting to further boost ketone production. And to really increase BHB production and fat burning, you can combine a ketogenic diet, intermittent fasting and exogenous ketones like BHB salts.
Is beta hydroxybutyrate safe BHB is something that your body produces to ensure survival in times of food scarcity or starvation. The production of ketones is actually a survival mechanism and totally natural and safe. However, taking exogenous ketones is a bit different, since these are not made by the body. Ketosis itself can also result in side effects, especially when you initially transition into this metabolic state.
Beta hydroxybutyrate side effects (or side effects of ketosis) may include unpleasant taste in your mouth, fatigue, weakness, indigestion, dizziness, low blood sugar, sleep-related issues, mood changes, frequent urination, constipation, cramps, and trouble exercising or recovering.
I would like to test beta hydroxybutyrate and am quite insterested in your kit (ab83390). From the protocol, I found dehydrogenase was used to oxidize hydroxybutyrate first and then a detection reagent will recognize oxobutyrate.Here, I am wondering whether the detection is specific for oxobutyrate. If there are any compounds in the mixture contains ketone, will they be detected also
The detection agent does not quantify the oxobutyrate but the byproduct of the reaction by the dehydrogenase. The reaction byproducts are produced proportionally based on the amount of beta hydroxybutyrate in a specific sample. This is why this assay kit is specific for beta hydroxybutyrate and will not recognize other ketones.
Thank you for your enquiry and interest in our products. I can confirm that this kit (ab83390: beta Hydroxybutyrate (beta HB) Assay Kit) measuresonly the β-Hydroxybutyrate in the samples. Hope this information has been helpful for you.
I have order two Abcam kits to measure beta-HB and Ammonia in mouse serum sample--beta Hydroxybutyrate (beta HB) Assay Kit (ab83390) and Ammonia Assay Kit (ab83360). Do you have any special suggestions on how to prepare blood sample
For serum or plasma samples, there are no special requirements for preparation of serum or plasma samples for the ammonia assay. For the beta HB assay, it is recommended (see section 4 of the protocol) that samples be de-proteinated with a 10 kDa filter, which we have as item ab93349.
using the ab93349 assay kit to detect hydroxybutyrate. I understand that the enzyme provided in the kit is the hydroxybutyrate dehydrogenase. In order for the kit to work, is the assumption made that the biological fluid being analyzed has the diaphorase that oxidizes the NADH and transfers electrons to the detection dye We are simply trying to better understand the principle of the technique and how to make sure we are including appropriate controls. 59ce067264